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Do you have something on the hot shoe? If so, take it off.
This is a crazy Canon design fault, The spring clips in the shoe mount are pressed down, and dont relase upwards when you take your mic or light off. To remedy this,
simply prise up the clips with a toothpick or similar and your flash wil work again. Tip. Always use a bracket to mount accesories on the camera.
Hope this helps
The little metal spring strip at the bottom of the shoe, (1 each side), they depress down when you mount anything on the shoe. simply prise them both up, just a little, and the problem will be solved.
I was trying to keep it simple. There is a small pin under the clip that gets stuck down, it has to be released for the flash to work. Running a blade or toothpck under the spring strip is normally enough to release it. try Googling " 550D flash wont pop up" or something similar, it will give you all the answers you need.
Check this out for full explanation of the problem, and the remedy. http://www.photomalaysia.com/forums/showthread.php?t=136213